|
KMID : 1143420200130322383
|
|
Public Health Weekly Report
2020 Volume.13 No. 32 p.2383 ~ p.2391
|
|
|
Laboratory Diagnostic Test of Human Granulocytic Anaplasmosis in Korea, 2019
|
|
Kim Hwan-Hee
Lee Kyeong-Ah
Hwang Seon-Do
Chun Jeong-Hoon
Hwang Kyu-Jam
|
|
|
Abstract
|
|
|
|
Human granulocytic anaplasmosis (HGA) is a zoonotic tick-borne disease caused by Anaplasma phagocytophilum (A. phagocytophilum), which is transmitted to humans by tick bites. Typical symptoms include high fever, headaches, chills and muscle aches occurring within 1-2 weeks of a tick bite. This study presented the results of the laboratory diagnosis of 1,568 cases of HGA in suspected patients in 2019. This study performed serological tests for HGA using indirect immunofluorescent antibody assay (IFA) using the sera of 1,096 patients and amplification of the 16S rRNA gene of A. phagocytophilum by nested polymerase chain reaction (PCR) of 472 blood samples. As a result of the IFA test, 197 (18.0%) of the 1,096 cases were reactive for Immunoglobulin G (IgG) or Immunoglobulin M (IgM) against A. phagocytophilum. In terms of participants, there were 103 males (52.3%) and 94 females (47.7%), and 139 cases were aged 50 years and over (70.6%). The serum response rates in 2018 (21.3%) and 2019 (18.0%) were compared to 2015 (7.0%), 2016 (5.3%), and 2017 (9.4%). In the last 5 years of serological testing, it was confirmed that the increase in serum response rate was more than 10%. In addition, nineteen out of 472 genetic PCR tests (4.0%) showed positive results. Either the antibody titer in the recovery serum was more than four times higher than that in the acute serum, or the IgG value was 1:320 or higher, or gene detection was judged as diagnostically positive. According to the laboratory diagnostic criteria, the positive rate of HGA was 1.7% (5/302 cases) in 2016 and 5.0% (30/598 cases) in 2017, 6.6% (80/1,214 cases) in 2018 and 2.7% (43/1,568 cases) in 2019. Based on these results, this study concluded that laboratory diagnosis tests for suspected patients are required. Furthermore, this study recommended that disease precautions during outdoor activities must be taken to prevent HGA.
|
|
|
KEYWORD
|
|
|
Human granulocytic anaplasmosis (HGA), Anaplasmosis, Anaplasma phagocytophilum, Laboratory diagnosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|